|
KMID : 0377619720220040441
|
|
Korean Jungang Medical Journal
1972 Volume.22 No. 4 p.441 ~ p.449
|
|
|
Vibriocidal Activity of Anti-cholera Serum
|
|
|
|
|
Abstract
|
|
|
Gram-negative bacilli usually survive longer in their homologous antisera than in normal serum, so far as the serum is not too diluted and the agglutinin titer is maintained above a certain level. The typical inhibitory role of antibodies in serum bactericidal activity has been well demonstrated by brucella, some of the salmonella, and E. coli strains.
In the present experiment, however, in contrast with such a seemingly contradictory attitude of antiserum, Vibrio cholerae antibodies were shown to have an entirely different effect on the serum bactericidal activity. The followings are the results obtained after studying comparatively the vibriocidal and cross-agglutinating reactions of cholera anti-serum.
1. Vibrio eholerae serotype Inaba and Ogawa, Brucella melitensis and abortus, and Salmonella typhi, enteritidis and thompson were employed as test organisms. Cells were suspended in saline solution at the rate of 2 x 104 /ml. 0. 5 ml of this suspension was put into the test tubes containing the same amount of variously diluted serum. The cell and serurni mixtures were incubated at 37°G for 3 hrs. And the number of survived cells was counted by the pour-plate method.
2. At the initial dilution of serum, no Cholera bacillus was detected surviving such treatments in cholera antiserum, while in normal serum considerable numbers of cell (100-500) were found surviving. As the serum was further diluted, however, the cell survival rate was increased gradually, regardless of the kind of serum.
3. On the other hand, in brucella and salmonella typhi, immune bodies seemed to have a strong inhibitory effect on the serum bactericidal action. Therefore, the less diluted the original serum, the more numbers of cell survived in the immune serum while the less in normal one.
4. A moderate degree of cross agglutination was observed between Brucella abortus and Inaba strains. A similar reaction was also observed between Ogawa and brucella strains. But the
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|